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Simultaneous determination of some antibiotics with coccidiosis effect in feed by LC-MS/MS

Kieu Thi Lan Phuong Nguyen Thi Phuong Mai Dang Thu Hien Hoàng Thị Lan Anh Nguyen Thi Anh Huong
Published 12/27/2022

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How to Cite
Kieu Thi Lan Phuong, Nguyen Thi Phuong Mai, Dang Thu Hien, Hoàng Thị Lan Anh, Nguyen Thi Anh Huong. "Simultaneous determination of some antibiotics with coccidiosis effect in feed by LC-MS/MS". Vietnam Journal of Food Control. vol. 6, no. 1, pp. 1-11, 2022

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Liquid chromatography tandem mass spectrophotometry (LC-MS/MS) has been developed for simultaneous determination of four antibiotics which have coccidiicidal effects including monensin, dimetridazole, sulfadimethoxin, and sulfadimidine in feed samples. The research conducted by using mobile phase A (0.1% formic acid solution; 10 mM ammonium formate) and phase B (MeOH; 0.1% formic acid; 10 mM ammonium formate) with C18 reversed-phase chromatography column (2.1 × 1.5 mm, ID 3.5 μm) simultaneously and successfully identified these four antibiotics in a short time about 10 minutes. The method detection limits and quantification limits for all analytes were 15.0 and 45.0 µg/kg, respectively. The calibration curve of the method was in the range of 45 - 1,500 µg/L. The method was validated for high accuracy, efficiency and applied to analyze the feed samples on the Hanoi market.


Monensin, Dimetridazole, Sulfadimidine, Sulfadimethoxine, coccidiosis, LC-MS/MS.


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