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Development of a high-performance liquid chromatography method for simultaneously analyzing Guanosine 5’-monophosphate (GMP) and Inosine 5’-monophosphate (IMP) in food

Published 12/24/2021

Article Details

How to Cite
Le Dinh Hai, Nguyen Thi Thu, Vu Thi Trang, Le Thi Thuy. "Development of a high-performance liquid chromatography method for simultaneously analyzing Guanosine 5’-monophosphate (GMP) and Inosine 5’-monophosphate (IMP) in food". Vietnam Journal of Food Control. vol. 4, no. 4 (en), pp. 298-306, 2021
PP
298-306
Counter
406

Main Article Content

Abstract

This study aimed to develop a HPLC method to simultaneously analyze guanosine 5’-monophosphat (GMP) and inosine 5’-monophosphat (IMP) in food products. Sample preparation procedure was simple, fast. A C18 column (250 mm × 4.6 mm, 5 µm) was used as stationary phase, and a mixture of 10 mM potassium dihydrogen phosphate and 5 mM sodium heptanesulfonate was applied as mobile phase, and PDA detector at 250 nm. The method validation followed AOAC criteria. Selectivity, linearity (R2 > 0.999), recovery (IMP: 90.5 % - 102.8 %, GMP: 91.5 % - 103.9 %), repeatability (RSDR of IMP: 3.07 % and GMP: 2.83 %) were acceptable to determination GMP and IMP in food matrix under AOAC guidelines. LOD of GMP and IMP were of 2.32 and 2.77 mg/kg, respectively. This method was used to determination GMP, IMP in food products collected in Hanoi markets.

Keywords:

GMP, IMP, HPLC, food matrix

References

[1]. Qiu W, Chen S, Xie J, Qu Y, and Song X, “Analysis of 10 nucleotides and related compounds in Litopenaeus vannamei during chilled storage by HPLC-DAD,” LWT Food Science and Technology, vol. 67, pp.187-193, 2016.
[2]. Ministry of Healh, “QCVN 4:1-23:2010/BYT”, 2010.
[3]. V. P. H. Dyta, “Analysis of disodium 5’-guanylate and disodium 5’-inosinate as flavor enhancer in food spices by thin-layer chromatography-densitometry,” International Journal of Applied Pharmaceutics, vol. 10, no. 1, pp.51, 2018.
[4]. R. A. F. Pereira, C. Ugucione, and E. R. Pereira-Filho, “Determination of inosine 5’-monophosphate (IMP) and guanosine 5’-monophosphate (GMP) in yeast extracts using UV spectroscopy and chemometrics,” Brazilian Journal of Food Technology, vol. 21, 2018.
[5]. D. K. Natalia and T. I. Rukmana, “Development of a high-performance liquid chromatography method for analyzing disodium 5′-guanylate and disodium 5′-inosinate levels in flavor enhancers,” International Journal of Applied Pharmaceutics, vol. 10, no. 1, pp.133, 2018.
[6]. L. Coulier, R. Bas, M. Hekman, V. D. Werff, M. Burgering, and U. Thissen, “Comprehensive Analysis of Umami Compounds by Ion-Pair Liquid Chromatography Coupled to Mass Spectrometry,” Journal of Food Science, vol. 76, no. 7, pp. 1081-1087, 2011.
[7]. G. Xiao, H. Xiao, Y. Zhu, and Y. You, “Determination of nucleotides in Chinese human milk by high-performance liquid chromatography-tandem mass spectrometry,” Dairy Science & Technology, vol. 94, no. 6, pp. 591-602, 2014.
[8]. T. C. Son, “Method validation in chemical and microbiological analysis”, Ha Noi: Science and Technics Publishing House, 2016.
[9]. AOAC Official Methods of Analysis, Guidelines for Standard Method Performance Requirements, Appendix F, 2016.

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