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Developing an electrochemical aptasensor using a specific aptamer to detect neomycin antibiotic in milk

Nguyen Truong Giang Le Quang Huan
Received: 25 Jul 2021
Revised: 27 Sep 2021
Accepted: 27 Sep 2021
Published: 30 Sep 2021

Article Details

How to Cite
Nguyen Truong Giang, Le Quang Huan. "Developing an electrochemical aptasensor using a specific aptamer to detect neomycin antibiotic in milk". Vietnam Journal of Food Control. vol. 4, no. 3, pp. 239-250, 2021
PP
239-250
Counter
795

Main Article Content

Abstract

Milk and dairy products, often considered healthy and nutritionally balanced natural foods, include essential nutrients for all ages and are an important part of the daily diet. However, the indiscriminate use of antibiotics, more than the allowable dose, and improper purposes makes food and animal products in general and dairy at risk of harming human health. Sensitive consumers may experience allergies or even more severe health problems. Antibiotics are left over for a long time, and it will lead to antibiotic resistance, making it difficult for people to control diseases and causing many consequences in the future. There are many modern methods proposed to detect antibiotics in food. One of the main methods is the electrochemical method. In this study, we used aptamer Neo6 and gold nanochip to fabricate an electrochemical aptasensor capable of determining neomycin residues in milk with a linear range of 10 - 3,000 ng/mL as determining the accuracy of the neomycin residues in milk. The sensitivity and specificity of the aptasensor are quite high. In addition, we initially compared over 20 milk samples supplemented with antibiotics, showing similar results with those analyzed by LC-MS/MS method.

Keywords:

aptamer Neo6, electrochemistry, neomycin, gold electrode, aptasensor.

References

[1]. S. A. Waksman, and H. A Lechevalier, "Neomycin, a New Antibiotic Active against Streptomycin-Resistant Bacteria, including Tuberculosis Organisms," Science, vol. 109, pp. 305-307, 1949.
[2]. GT Dow, JB Thoden, and HM Holden, "The three‐dimensional structure of NeoB: An aminotransferase involved in the biosynthesis of neomycin," Protein Sciemce, vol. 27, pp. 945-956, 2018.
[3]. C. Manyi-Loh, S. Mamphweli, E. Meyer, and A. Okoh, "Antibiotic Use in Agriculture and Its Consequential Resistance in Environmental Sources: Potential Public Health Implications," Molecules, vol. 23, pp. 795, 2018.
[4]. T. Kusano, M. Kanda, K. Kamata, and T. Miyazaki, "Microbiological method for the detection of antibiotic residues in meat using mixed-mode, reverse-phase and cation-exchange cartridge,” Shokuhin Eiseigaku Zasshi, vol. 45, pp. 191–196, 2004
[5]. S. Ahmed, J. Ning, D. Peng, T. Chen, et al., "Current advances in immunoassays for the detection of antibiotics residues: a review," Food and Agricultural Immunology, vol.31, pp. 268-290, 2020.
[6]. C. Yan, J. Zhang, L. Yao, et al., "Aptamer-mediated colorimetric method for rapid and sensitive detection of chloramphenicol in food," Food Chem, vol. 260, pp. 208-212, 2018
[7]. R. Mirzaei, M. Yunesian, S. Nasseri, et al., "An optimized SPE-LC-MS/MS method for antibiotics residue analysis in ground, surface and treated water samples by response surface methodology - central composite design," Journal of Environmental Health Science & Engineering, vol. 15, pp. 21, 2017.
[8]. A. D. Keefe, S. Pai, and A. Ellington, "Aptamers as therapeutics," Nature Review. Drug Discovery, vol. 9, no. 7, pp. 537-550, 2010.
[9]. N. R. Ha, I. P. Jung, I. J. La, H. S. Jung, and M. Y. Yoon, "Ultra-sensitive detection of kanamycin for food safety using a reduced graphene oxide-based fluorescent aptasensor," Scientific Reports, vol. 7, pp. 40305, 2017.
[10]. Z. Li, M. A. Mohamed, A. M. Vinu Mohan, et al., "Application of Electrochemical Aptasensors toward Clinical Diagnostics, Food, and Environmental Monitoring: Review," Sensors (Basel), vol. 19, pp. 5435, 2019.
[11]. D. Sharma, J. Lee, J. Seo, and H. Shin, "Development of a Sensitive Electrochemical Enzymatic Reaction-Based Cholesterol Biosensor Using Nano-Sized Carbon Interdigitated Electrodes Decorated with Gold Nanoparticles," Sensors, vol. 17, pp. 2128, 2017
[12]. A. Salek Maghsoudi, S. Hassani, and M Rezaei Akmal, "An Electrochemical Aptasensor Platform Based on Flower-Like Gold Microstructure-Modified Screen-Printed Carbon Electrode for Detection of Serpin A12 as a Type 2 Diabetes Biomarker," InternationalJournal of Nanomedicine, vol. 15, pp. 2219-2230, 2020
[13]. Hệ thống sắc ký lỏng khối phổ hai lần LC-MS/MS, [Trực tuyến]. Địa chỉ https: //nifc.gov.vn/index.php/vi/tblvhh/1458-ha-thang-sac-ka-lang-khai-pha-hai-lan-lc-ms-ms [Truy cập 27/9/ 2021].
[14]. S. Ghoneim, Accuracy, Recall, Precision, F-Score & Specificity, which to optimize on?, Medium. https://towardsdatascience.com/accuracy-recall-precision-f-score-specificity-which-to-optimize-on-867d3f11124 (accessed September 24, 2021).
[15]. H. Zhu, X. Chen, Z. Zheng, X. Ke, E. Jaatinen, J. Zhao, C. Guo, T. Xie,, and D. Wang et al., "Mechanism of supported gold nanoparticles as photocatalysts under ultraviolet and visible light irradiation," Chemical Communications, pp. 7524-7526, 2009.
[16]. F. Li, X. Gao, X. Wang, et al., "Ultrasensitive sandwich RNA-aptasensor based on dual-signal amplification strategy for highly sensitive neomycin detection," Food Control, vol. 131, pp. 108445, 2022
[17]. Thông tư 24/2013/TT-BYT mức giới hạn tối đa dư lượng thuốc thú y, [Trực tuyến]. Địa chỉ https://thuvienphapluat.vn/van-ban/Bo-may-hanh-chinh/Thong-tu-24-2013-TT-BYT-muc-gioi-han-toi-da-du-luong-thuoc-thu-y-204380.aspx [Truy cập 27/9/ 2021].

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