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Determination of myo-inositol content in milk by high performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD)

Published 09/16/2021

Article Details

How to Cite
Le Viet Ngan, Do Thi Hong Thuy, Nguyen Thi Anh Huong, Vu Thi Trang, Le Thi Hong Hao. "Determination of myo-inositol content in milk by high performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD)". Vietnam Journal of Food Control. vol. 4, no. 3, pp. 207-218, 2021
PP
207-218
Counter
472

Main Article Content

Abstract

A method to determine inositol content in milk using high performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) was developed and validated. Samples were hydrolyzed with 10 mL HCl in a vacuum oven at 120oC, prolonging 6 h. Hydrolyzed samples were neutralized to a pH from 6 to 7.5 before analysing in an HPAEC-PAD system. The analytical program used Dionex CarboPacTM MA1 column (4 × 250 mm) with a mobile phase of a gradient program including NaOH 50 mM and NaOH 1M. The method was validated following AOAC guidelines: selectivity, linear range from 0,01 to 20 mg/L with a coefficient (R) 0.9998, the recoveries in the range of 99 - 102%, and repeatability with RSD under 1.8%. The limit of detection (LOD) and the limit of quantification (LOQ) of inositol were 0.047 µg/g and 0.155 µg/g, respectively. The method has been applied to determine of inositol content in milk samples with content ranging from 22.5 mg/100g to 64.7 mg/100g.

Keywords:

myo-inositol, high performance anion exchange chromatography, HPAEC, PAD.

References

[1]. J. Levine, , “Controlled trials of inositol in psychiatry,” European Neuropsychopharmacology, vol.7 no. 2, pp. 147–155, 1977.
[2]. A. I. Barkai, D. L. Dunner, H. A. Gross, P. Mayo, and R. R. Fieve.“Reduced myo-inositol levels in cerebrospinal fluid from patients with affective disorder,” Biology Psychiatry, vol. 13, no.1, pp. 65-72, 1978.
[3]. H. Shimon, G. Agam, R. H. Belmaker, T. M. Hyde, and J. E. Kleinman. “Reduced frontal cortex inositol levels in postmortem brain of suicide victims and patients with bipolar disorder,” American Journal of Psychiatry, vol. 154, no. 8, pp. 1148-1150, 1997.
[4]. F. Fruzzetti, , D. P erini, M. Russo, F. Bucci, and A. Gadducci “Comparison of two insulin sensitizers, metformin and myo-inositol, in women with polycystic ovary syndrome (PCOS),” Gynecological Endocrinology, vol. 33, no. 1, pp. 39-42, 2016.
[5]. A. D. Genazzani, C. Lanzoni, F. Ricchieri, and V. M. Jasonni “Myo-inositol administration positively affects hyperinsulinemia and hormonal parameters in overweight patients with polycystic ovary syndrome,” Gynecological Endocrinology, vol. 24, no. 3, pp. 139-144, 2008.
[6]. A. Howlett, A. Ohlsson, and N. Plakkal, “Inositol for respiratory distress syndrome in preterm infants,” The Cochrane database of systematic reviews. (3): CD000366, 2012.
[7]. B. M. Simonet, A. Ríos, F. Grases, and M. Valcárcel “Determination of myo-inositol phosphates in food samples by flow injection-capillary zone electrophoresis,” Electrophoresis, vol. 24, no. 12-13, pp. 2092-2098, 2003.
[8]. J. Guo, Y. Shi, C. Xu, R. Zhong, F. Zhang, T. Zhang, and , J. Wang “Quantification of plasma myo-inositol using gas chromatography–mass spectrometry,” Clinica Chimica Acta, vol. 460 pp. 88-92, 2016.
[9]. O. P. S. Rebecca, A. N., Boyce, and C. Somasundram, “Isolation and Identification of Myo-Inositol Crystals from Dragon Fruit (Hylocereus polyrhizus),” Molecules, vol. 17, no. 4, pp. 4583-4594, 2012.
[10]. J.-H., Shin, J.-M.Park, H.-J., Kim, J.-H., Ahn, B.-M., Kwak, and J.-M. Kim, “Development Rapid Analytical Methods for Inositol as a Trace Component by HPLC and LC-MS/MS in Infant Formula,”Korean Journal for Food Science of Animal Resources, vol. 35, no. 4, pp. 466–472, 2015.
[11]. D. Ellingson, T. Pritchard, P. Foy, K. King, B. Mitchell, J. Austad, D. Winters, D. Sullivan, “Analysis of Free and Total Myo-Inositol in Foods, Feeds, and Infant Formula by High-Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, including a Novel Total Extraction Using Microwave-Assisted Acid Hydrolysis and Enzymatic Treatment”, Journal of AOAC International, vol. 95, no. 5, pp. 1469–1478, 2012.
[12]. G. Carlomagno, V. Unfer ,“Inositol safety: clinical evidences,” European Review for Medical and Pharmacological Sciences, vol. 15, no. 8 pp. 931-936, 2011.

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