Smilax glabra rhizoma (SGR) has been used in traditional medicine remedies for the treatment of joint pain and inflammation, and recently, has been presented in dietary supplements for supporting arthritis and gout treatment. In the 5th Vietnamese Pharmacopoeia, astilbin was chosen as a chemical marker for the quality control of Smilax glabra as herbal medicine. A high performance liquid chromatographic (HPLC) method was developed for the qualitative and quantitative determination of astilbin in dietary supplements containing Smilax glabra. Ultrasonic extraction with 60% methanol was used for astilbin extraction from herbal products. HPLC analysis was performed with a C18 column and a mobile phase consisting of methanol and water in gradient mode, with UV detection at 291 nm. The method was validated according to AOAC International’s requirements with high specificity and precision.
astilbin, Smilax glabra, dietary supplement, high performance liquid chromatography, HPLC.
. D. H. Bich, D. Q. Chung, B. X. Cương, N. Thuong Dong, D. T. Dam, P. V. Hien, V. N. Lo, P. D. Mai, P. K. Man, D. T. Nhu, N. Tap, and T. Toan, “Medicinal plants and animals in Vietnam,” Hanoi: Science and Technology Publisher, vol.2, pp. 883-886, 2003.
. L. Huang, J. Deng, G. Chen, M. Zhou, J. Liang, B. Yan, J. Shu, Y. Liang, and H. Huang, “The anti-hyperuricemic effect of four astilbin stereoisomers in Smilax glabra on hyperuricemic mice,” Journal of Ethnopharmacology, vol. 238: 111777, 2019.
. C-L. Lu, Y-F. Zhu, M-M. Hu, D-M. Wang, X-J Xu, C-J. Lu, and W. Zhu, “Optimization of astilbin extraction from the rhizome of Smilax glabra, and evaluation of its antiinflammatory effect and probable underlying mechanism in lipi-polysaccharide-induced RAW264.7 macrophages,” Molecules, vol. 20, no. 1, pp. 625-644, 2015.
. M. Huong, “Research on biological activities and chemical compositions in the roots of Smilax glabra rhizoma (SMILAX GLABRA ROXB.) in Vietnam,” Master’s Thesis in Experimental Biology, Institute of Ecology and Biological Resources, 2013.
. P. T. H. Minh, N. Q. Tien, T. T. T. Hang, and P. H. Dien, “Research on the chemical composition of the Smilax glabra Roxb. grown in Thai Nguyen,” Journal of Science, Hanoi National University of Education, vol 55, no. 6, pp. 62-70, 2010.
. T. T. T. Van, V. V. Chien, P. T. Hang, P. V. Cuong, N. Q. Vuong, “Antioxidant activity of extracts and astilbin from the root of Smilax glabra of Vietnam,” Malaysian Journal of Chemistry, 17, 12-19, 2015.
. Pharmacopoeia Council - Ministry of Health, Vietnam Pharmacopoeia V, Medicine Publisher, vol. II, pp. 1344-1345, 2017.
. L. V. Duc, V. B. Duong, T. N. Trung, and P. T. T. Huong, “Quantitative study for determination of astilbin in Smilax glabra by high performance liquid chromatography,” Journal of Military Pharmaco-Medicine, vol. 7, pp. 8-12, 2015.
. AOAC International, “Appendix K: Guidelines for dietary supplements and botanicals,” AOAC Official Methods of Analysis, pp. 3-32, 2013.
. Qing-Feng Zhang, Ying-Juan Fu, Zhan-Wang Huang, Xin-Cheng Shangguang, Yu-Xian Guo, “Aqueous Stability of Astilbin: Effects of pH, Temperature, and Solvent,” Journal of Agricultural and Food Chemistry, 61 (49), 12085-12091, 2013.