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Simultaneous determination of borax and polyphosphates content in food by liquid chromatography inductively coupled plasma mass spectrometry (LC-ICP-MS)

Dinh Viet Chien Dang Thi Hien Nguyen Minh Chau Le Van Ha Tran Hoang Giang Lu Thi Minh Hien Nguyen Van Khoa Pham Gia Bach Le Thi Hong Hao
Received: 08 Apr 2021
Revised: 28 May 2021
Accepted: 28 May 2021
Published: 30 Jun 2021

Article Details

How to Cite
Dinh Viet Chien, Dang Thi Hien, Nguyen Minh Chau, Le Van Ha, Tran Hoang Giang, Lu Thi Minh Hien, Nguyen Van Khoa, Pham Gia Bach, Le Thi Hong Hao. "Simultaneous determination of borax and polyphosphates content in food by liquid chromatography inductively coupled plasma mass spectrometry (LC-ICP-MS)". Vietnam Journal of Food Control. vol. 4, no. 2 (en), pp. 115-126, 2021

Main Article Content


A new method based on LC-ICP-MS technique has been developed and validated for the simultaneous determination of borax and polyphosphates (phosphate, pyrophosphate, tripolyphosphate) in foods. The analytes were extracted from the samples with 18 mM sulfuric acid solution using ultrasonic vibration extraction technique before being filtered and analyzed by LC-ICP-MS system. The method was validated with calibration curves that have the coefficient of determination (R2) higher than 0.995. The method limits of detection (LOD) of borax, phosphate, pyrophosphate (Pyro-P), and tripolyphosphate (Tripoly-P) were in the range of 2.5 to 15.0 mg/kg. The recoveries were in the range of 86.0 to 107.0% and the relative standard deviations were below 5.23%. A total of 83 samples of cereal, meat, and seafood-based food were analyzed for research purposes. The results did not indicate a risk of using borax and polyphosphates in most rice products. Meanwhile, meat products including pork paste and roasted pork sold in traditional markets had a borax detection rate of over 60%. This study showed that there was still a risk of using borax in food processing and the use of polyphosphates in food products collected in some districts in Hanoi city.


borax, borate, polyphosphate, pyrophosphate, tripolyphosphate, food, LC-ICP-MS.


[1]. “Borax (Na2B4O7.10H2O) - Sodium Borate - Occurrence, Discovery and Applications,” Amoz.com. August 16, 2004.
[2]. The Circular 24/2019/TT-BYT, Regulations on management and use of food additives.
[3]. Thanh Tung, Formol is still rampant in pho - Borax is still spreading in spring rolls, https://thanhnien.vn/suc-khoe/formol-van-tran-lan-trong-pho-han-the-van-tran-lan-tronggio-cha-325759.htmL, 2006.
[4]. D. T. Hoa, N. T. K. Dung, and T. X. Bach, “The situation of using borax and illegal food coloring in some processed foods at some markets in Dong Da and Hai Ba Trung districts - Hanoi 2005,” Journal Practical Medicine, no. 10, 2007.
[5]. Quang Tri Sub-Department of Food Safety and Hygiene, “The issue of using borax in food,” http://antoanthucpham.quangtri.gov.vn/Tin-tuc-su-kien/Van-de-su-dung-han-thetrong-thuc-pham-16.html, December 19, 2012.
[6]. T. H. Hoai, “Evaluation of food contamination in Dak Lak province from 2014 - 2018,” Vietnamese Journal of Food Control. vol. 2, no. 3, pp. 44-50, 2019.
[7]. N. Duong, Ha Nam, “Fined VND 40 million, temporarily suspending production facilities using solder,” http://daidoanket.vn/an-toan-thuc-pham/ha-nam-phat-40-trieu-dong-tamdinh-chi-co-so-san-xuat-gio-su-dung-han-the-tintuc456124, January 4, 2020.
[8]. B. Nhung, “Powerless to control welding used to process and preserve food, https://baohatinh.vn/y-te/bat-luc-trong-kiem-soat-han-the-dung-che-bien-bao-quan-thuc-pham/148008.htm, January 17, 2018.
[9]. AOAC Official Method 969.26, Spectrophotometric Method - Boric Acid in Caviar.
[10]. AOAC Official Method 972.19, Atomic Absorption Spectrophotometric Method – Boric Acid in Food.
[11]. AOAC Official Method 975.26, Emission Spectroscopic Method - Boric Acid in Food.
[12]. Akinobu Matsunaga, Atsushi Yamamoto, Eiichi Mizukami, and Tooru Ooizumi, “Determination of Polyphosphates in Foods by High Performance Liquid Chromatography,” Published by Japanese Society for Food Science and Technology, vol. 37, no. 1, pp. 20-25, 1990.
[13]. Marco Iammarino, Aurelia Di Taranto, “Determination of polyphosphates in products of animal origin: application of a validated ion chromatography method for commercial samples analyses,” European Food Research and Technology, vol. 235, pp. 409-417, 2012.
[14]. N. T. Lien, N. T. A. Huong, C. V. Hoang, P. Q. Trung, and L. T. H. Hao, “Study on the process of simultaneous analysis of pyrophosphate and triphosphate in some food samples by capillary electrophoresis using non-contact conductivity detector (CE-C4D),” Journal of Analytical Chemistry, Physics and Biology, vol. 23, no. 1, pp. 121-127, 2018.
[15]. Thermo Scientific, Determination of polyphosphates using ion chromatography, Application update 172, 2016.
[16]. Shodex, Application search, analysis of pyrophosphate and tripolyphosphate [online], https://www.shodex.com/en/dc/07/06/15.html [Accessed date: 15/12/2020].
[17]. ISO 5553:1980. Meat and meat products - Detection of polyphosphates.