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Determination of esomeprazole in rabbit plasma by liquid chromatography tandem mass spectrometry (LC-MS/MS)

Le Minh Anh Nguyen Thach Tung Nguyen Nhu Nam Bui Quang Dong Pham Thanh Dat Nguyen Thi Hong Ngoc Tran Cao Son
Published 03/15/2021

Article Details

How to Cite
Le Minh Anh, Nguyen Thach Tung, Nguyen Nhu Nam, Bui Quang Dong, Pham Thanh Dat, Nguyen Thi Hong Ngoc, Tran Cao Son. "Determination of esomeprazole in rabbit plasma by liquid chromatography tandem mass spectrometry (LC-MS/MS)". Vietnam Journal of Food Control. vol. 4, no. 1, pp. 10-21, 2021
PP
10-21
Counter
752

Main Article Content

Abstract

A rapid extraction method was developed and validated for esomeprazole determination in rabbit plasma by liquid chromatography tandem mass spectrometry (LC–MS/MS). Esomeprazole in rabbit plasma was extracted and then cleaned up using QuEChERS technique. The analyte was separated using C18 column and quantified by MS/MS detector. Pantoprazole was used as the internal standard. The positive ESI source was used in this study together with the multi­-reactive ion monitoring mode. The validity of the method has been confirmed in accordance with ICH Harmonized guidelines on bioanalytical method validation. The method showed good specificity, good stability, with the linearity varying from 0.1 ng/mL to 20 ng/mL, the lower limit of quantification was 0.1 ng/mL, the accuracy and precision were within 85% and 115% which achieved the ICH Harmonized guideline requirements, FDA guideline and AOAC International requirements. The method has been applied to quantify the concentration of esomeprazole in rabbit plasma, and then to compare the bioavailability of two preparations containing esomeprazole.

Keywords:

Esomeprazole, Pantoprazole, LC-MS/MS, rabbit plasma, QuEChERS

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