A rapid extraction method was developed and validated for esomeprazole determination in rabbit plasma by liquid chromatography tandem mass spectrometry (LC–MS/MS). Esomeprazole in rabbit plasma was extracted and then cleaned up using QuEChERS technique. The analyte was separated using C18 column and quantified by MS/MS detector. Pantoprazole was used as the internal standard. The positive ESI source was used in this study together with the multi-reactive ion monitoring mode. The validity of the method has been confirmed in accordance with ICH Harmonized guidelines on bioanalytical method validation. The method showed good specificity, good stability, with the linearity varying from 0.1 ng/mL to 20 ng/mL, the lower limit of quantification was 0.1 ng/mL, the accuracy and precision were within 85% and 115% which achieved the ICH Harmonized guideline requirements, FDA guideline and AOAC International requirements. The method has been applied to quantify the concentration of esomeprazole in rabbit plasma, and then to compare the bioavailability of two preparations containing esomeprazole.
Esomeprazole, Pantoprazole, LC-MS/MS, rabbit plasma, QuEChERS
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