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Simultaneous determination of Nisin A and Nisin Z in nutritional food by liquid chromatography tandem mass spectrometry (LC-MS/MS)

Tran Thi Huong Huong Le Thi Thuy Vu Thi Trang Nguyen Thi Anh Huong Le Thi Hong Hao
Received: 16 Nov 2020
Revised: 05 Feb 2021
Accepted: 05 Feb 2021
Published: 31 Mar 2021

Article Details

How to Cite
Tran Thi Huong Huong, Le Thi Thuy, Vu Thi Trang, Nguyen Thi Anh Huong, Le Thi Hong Hao. "Simultaneous determination of Nisin A and Nisin Z in nutritional food by liquid chromatography tandem mass spectrometry (LC-MS/MS)". Vietnam Journal of Food Control. vol. 4, no. 1, pp. 43-51, 2021

Main Article Content


A method for the simultaneous determination of Nisin A and Nisin Z in nutritional products by LC-MS/MS has been developed. Sample treatment was performed by ultrasonic extraction using a mixture of 0.1 M CH3COONH4 buffer solution - 1.0 M NaCl (pH 2.0): MeOH (1:1, v/v) at room temperature for 10 minutes. Extracts were then cleaned through C18 solid phase extraction column (500 mg, 3 mL) and analyzed on LC-MS/MS instrument using a C18 column (100 mm × 2 mm × 1.7 µm) with ESI (+) mode. The method was validated for linear calibration curve in the range of 10 - 1,000 µg/L; relative standard deviation (RSD) 2.12 - 5.77%; recovery 80.1 - 105%, meeting AOAC requirements. The method has been applied to analyze 25 samples of nutritious food collected from the local markets (including: liquid milk, powdered milk, butter, cheese, nutritious powder), showing that two samples were detected both Nisin A and Nisin Z.


LC-MS/MS, Nisin A, Nisin Z, food, nutritional food


[1]. Thông tư 24/2019/TT-BYT, Quy định về quản lý và sử dụng phụ gia thực phẩm, Bộ Y tế, 2019.
[2]. J. W . Mulders, I. J. Boerrigter, H. S. Rollema, R. J. Siezen & W. M. de Vos, “Identification and characterization of the lantibioticnisin Z, a natural nisin variant”, European of Journal Biochemistry, vol. 201, no. 3, pp. 581-584, 1991.
[3]. Soliman, L.C. and K.K. Donkor, “Method development for sensitive determination of Nisin in food products by micellar electrokinetic chromatography”, Food Chemistry, 119(2), pp. 801- 805, 2010.
[4]. N. Schneider, K. Werkmeister, M. Pischetsrieder, “Separation and determination of closely related lantibiotics by micellar electrokinetic chromatography”, Journal of Separation Science, vol. 32, no. 17, pp. 2993-3000, 2009.
[5]. Tiêu chuẩn Việt Nam TCVN 10137:2013, Xác định hàm lượng Nisin A bằng sắc ký lỏng - phổ khối lượng (LC-MS) và sắc ký lỏng - phổ khối lượng hai lần (LC-MS-MS), Tổng Cục Tiêu chuẩn Đo lường Chất lượng thẩm định, Bộ Khoa học và Công nghệ công bố, 2013.
[6]. F. Fuselli, C. Guarino, A. L. Mantia, L. Longo, A. Faberi and R. M. Marianella, “Multi-detection of preservatives in cheeses by liquyd chromatography-tandem mass spectrometry”, Journal of Chromatography B, vol. 906, pp. 9-18, 2012.
[7]. K. Y. Ko, S. R. Park, C. A. Lee and M. Kim, “Analysis method for determination of Nisin A and Nisin Z in cow milk by using liquyd chromatography-tandem mass spectrometry”, Journal of Dairy Science, vol. 98, no. 3, pp. 1435-1442, 2015.
[8]. N. Schneider, K. Werkmeister and M. Pischetsrieder, “Analysis of Nisin A, Nisin Z and their degradation products by LCMS/MS”, Food Chemistry, vol. 127, no. 2, pp. 847-854, 2011.
[9]. W. Liu and J. N. Hansen, “Some chemical and physical properties of nisin, a small-protein antibiotic produced by Lactococcus lactis”, Applied and Environmental Microbiology, vol. 56, no. 8, pp. 2551 - 2558, 1990.
[10]. S. Suda, D. Field and N. Barron, “Antimicrobial peptide production and purification”, Protein Chromatography, Humana Press, New York, NY, pp. 401-410, 2017.
[11]. AOAC. Appendix F: Guidelines for standard method performance requirements. AOAC official methods of Analysis, 9, 2012.