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Simultaneous determination of Nisin A and Nisin Z in nutritional food by liquid chromatography tandem mass spectrometry (LC-MS/MS)

Tran Thi Huong Huong Le Thi Thuy Vu Thi Trang Nguyen Thi Anh Huong Le Thi Hong Hao
Received: 16 Nov 2020
Revised: 05 Feb 2021
Accepted: 05 Feb 2021
Published: 31 Mar 2021

Article Details

How to Cite
Tran Thi Huong Huong, Le Thi Thuy, Vu Thi Trang, Nguyen Thi Anh Huong, Le Thi Hong Hao. "Simultaneous determination of Nisin A and Nisin Z in nutritional food by liquid chromatography tandem mass spectrometry (LC-MS/MS)". Vietnam Journal of Food Control. vol. 4, no. 1, pp. 43-51, 2021
PP
43-51
Counter
948

Main Article Content

Abstract

A method for the simultaneous determination of Nisin A and Nisin Z in nutritional products by LC-MS/MS has been developed. Sample treatment was performed by ultrasonic extraction using a mixture of 0.1 M CH3COONH4 buffer solution - 1.0 M NaCl (pH 2.0): MeOH (1:1, v/v) at room temperature for 10 minutes. Extracts were then cleaned through C18 solid phase extraction column (500 mg, 3 mL) and analyzed on LC-MS/MS instrument using a C18 column (100 mm × 2 mm × 1.7 µm) with ESI (+) mode. The method was validated for linear calibration curve in the range of 10 - 1,000 µg/L; relative standard deviation (RSD) 2.12 - 5.77%; recovery 80.1 - 105%, meeting AOAC requirements. The method has been applied to analyze 25 samples of nutritious food collected from the local markets (including: liquid milk, powdered milk, butter, cheese, nutritious powder), showing that two samples were detected both Nisin A and Nisin Z.

Keywords:

LC-MS/MS, Nisin A, Nisin Z, food, nutritional food

References

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