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Determination of Phytosterols in vegetable oils by GC-MS method

Dang Thu Hien Mai Thi Ngoc Anh Nguyen Thi Hong Ngoc
Published 12/08/2020

Article Details

How to Cite
Dang Thu Hien, Mai Thi Ngoc Anh, Nguyen Thi Hong Ngoc. "Determination of Phytosterols in vegetable oils by GC-MS method". Vietnam Journal of Food Control. vol. 3, no. 4 (en), pp. 254-260, 2020
PP
254-260
Counter
770

Main Article Content

Abstract

In this study, a method for determination of six phytosterols by gas chromatography-mass spectrometry with derivatization in vegetable oils was validated. The samples were hydrolyzed in an alkaline media at 70°C for 60 min. Then, the samples were performed liquid-liquid extraction with toluene. The phytosterols are derivatized to trimethylsilyl ethers and then analyzed by gas chromatography-mass spectrometry. The limit of detection and limit of quantification was 5 and 15 mg/kg, respectively. Recoveries of six phytosterols were between 93.5% and 101%.

Keywords:

GC-MS, phytosterols, vegetable oils

References

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