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Simultaneous determination of methanol, ethanol and isopropanol in human blood and white spirit samples

Vu Anh Phuong Nguyen Thi Ngan Do Thi Trang Dinh Son Luong Nguyen Thi Anh Huong Hoang Quoc Anh
Received: 13 Nov 2020
Revised: 21 Dec 2020
Accepted: 21 Dec 2020
Published: 31 Dec 2020

Article Details

How to Cite
Vu Anh Phuong, Nguyen Thi Ngan, Do Thi Trang, Dinh Son Luong, Nguyen Thi Anh Huong, Hoang Quoc Anh. "Simultaneous determination of methanol, ethanol and isopropanol in human blood and white spirit samples". Vietnam Journal of Food Control. vol. 3, no. 4 (en), pp. 231-237, 2020

Main Article Content


A simple and cost-effective method for simultaneous determination of methanol, ethanol, and isopropanol in human blood and white spirit samples using headspace gas chromatography coupled with flame ionization detection (HS-GC-FID) was developed and validated for clinical and toxicological purposes. In this study, the headspace sampling procedure was investigated, indicating optimized temperature of 70 °C in 20 min. Concentrations of target compounds were determined by internal standard (IS) method with acetonitrile as IS compound. A good resolution of chromatographic peaks was achieved. The linear ranges for all the compounds were from 1 to 500 mg/dL. Our method was validated with adequate accuracy (recovery > 98%) and precision (RSD < 0.1%) in whole human blood and white spirit samples. The limits of detection were below 0.5 mg/L for the three compounds. This method is easy to perform, making it suitable for routine analysis in clinical biochemistry and forensic laboratories.


Methanol, ethanol, isopropanol, blood, white spirit, GC-FID


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