Bìa tạp chí

 

009bet

Prevalence of classical Staphylococcal enterotoxin genes of Staphylococcus aureus isolated from ready-to-eat food in Ho Chi Minh City, Vietnam

Nguyen Do Phuc Dang Thuy Linh Le Thi My Huong Tran Thi Thuy Nguyen Thi Ngoc Bong Quach Kim Ngoc
Received: 23 Oct 2020
Revised: 20 Dec 2020
Accepted: 20 Dec 2020
Published: 31 Dec 2020

Article Details

How to Cite
Nguyen Do Phuc, Dang Thuy Linh, Le Thi My Huong, Tran Thi Thuy, Nguyen Thi Ngoc Bong, Quach Kim Ngoc. "Prevalence of classical Staphylococcal enterotoxin genes of Staphylococcus aureus isolated from ready-to-eat food in Ho Chi Minh City, Vietnam". Vietnam Journal of Food Control. vol. 3, no. 4 (en), pp. 226-230, 2020
PP
226-230
Counter
947

Main Article Content

Abstract

Contamination of Staphylococcus aureus in ready-to-eat (RTE) food is a leading cause of foodborne illness in Vietnam. The aim of this study was to analyze the frequency of classical Staphylococcal enterotoxin genes (sea, seb, sec, sed, and see genes) in S. aureus strains isolated from ready-to-eat food in Ho Chi Minh, Vietnam (76 strains of  S. aureus were isolated from 200 random samples of ready - to - eat (RTE) food, which include pate, pork roll, barbecue pork, roasted pork and roasted duck, 40 samples of each), all the samples were collected from different fast food services on street in district 5, 6, 7 and 8 in Ho Chi Minh City, Vietnam. All the trains of S. aureus were stored at -70oC for analyzing Staphylococcal enterotoxin genes). The obtained results in this study indicated that out of 76 S. aureus strains, there are 6 strains (7.9%) carried SE genes:  sea gene (1.32%) was detected in one pate sample (Dist. 8), sec gene was detected (3.95%) in three samples, which are two pork roll samples (Dist. 7 and 8) and one roasted duck sample (Dist. 8), and finally see gene was detected in one pate sample (Dist. 8)and in one pork roll sample (Dist.  8). This could be a serious public health risk and highlight the need to implement good hygiene practices.

Keywords:

SE genes, S. aureus, Ready-to-eat food, Ho Chi Minh City

References

[1]. B. T. M. Huong, Z. H. Mahmud, S. B. Neogi, A. Kassu, N. V. Nhien, A. Mohammad, M. Yamato, F. Ota, N. T. Lam, H. T. A. Dao, N. C. Khan, “Toxigenicity and genetic diversity of Staphylococcus aureus isolated from Vietnamese ready-to-eat foods”, Medical Publisher, vol. 21, pp. 166-171, 2010.
[2]. E. D. Giannatale, V. Prencipe, A. Tonelli, C. Marfoglia and G. Mogliprati, “Characterisation of Staphylococcus aureus strains isolated from food fo human consumption”, Veterinaria Italiana, vol. 47, no.2, pp.165-173, 2011.
[3]. W. M. Johnson, S. D. Tyler, “PCR detection of genes for enterotoxins, Exfoliative toxins, and toxinshock syndrome toxin-1 in Staphylococcus aureus”, Diagnostic molecular microbiology, principles and application, pp. 294-299, 1991.
[4]. N. Đ. Phúc, N. T. H. Oanh, “Tỷ lệ nhiễm Staphylococcus aureus trong nhóm sản phẩm thịt sử dụng trực tiếp tại thành phố Hồ Chí Minh năm 2017, Tạp chí Phân tích Hóa, lý và Sinh học”, tập 23, số 5 (đặc biệt), tr. 117-121, 2018.
[5]. E. Perez-Roth, F. Claverie-Martin, J. Villar, S. Mendez-Alvarez, “Multiplex PCR for simultaneous identification of Staphylococcus aureus and detection of methicillin and mupirocin resistance”, Journal of Clinical Microbiology; vol. 39, no. 11, pp. 4037-4041, 2001.
[6]. V. L. M. Rall, J. M. Sfocin, V. C. M. Augustini, M. T. Watanabe, A. Jr. Fernandes, R. Rall, M. G Silva, J.P. Jr. Araujo, “Detection of enterotoxin genes of Staphylococcus sp. isolated form nasal cavities and hand of food handlers”, Brazilian Journal of Microbiology, vol. 41, no. 1, pp. 59-65, 2010.
[7]. Waranwan Wongboot, Chayriya Chomvarin and Wises Namwat, “Phenotypic and genotypic detection of enterotoxins, toxic shock syndrome toxin-1 and of methicillin resistance in Staphylococcus aureus isolated from retail ready to eat foods in northeastern Thailand”, The Southeast Asian Journal of Tropical Medicine and Public Health, vol. 46, no.1, pp. 97-104, 2015.
[8]. M. Yisalan, Z. Yanying, T. Junni, T. Cheng, C. Juan and L. Ji, “Antimicrobial susceptibility and presence of resistance and enterotoxin/enterotoxin-likes genes in Staphylococcus aureus from food”, Journal of Food, vol. 16, no.1, pp.76-84, 2017.

 Submit