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Screenning and determination of polyhydroxyalkanoate production by Bacillus sp. strains

Nguyen Thi Da Nguyen Trong Linh Nguyen Thu Trang Tran Manh Hai La Thi Huyen
Published 09/28/2020

Article Details

How to Cite
Nguyen Thi Da, Nguyen Trong Linh, Nguyen Thu Trang, Tran Manh Hai, La Thi Huyen. "Screenning and determination of polyhydroxyalkanoate production by Bacillus sp. strains". Vietnam Journal of Food Control. vol. 3, no. 3, pp. 165-172, 2020
PP
165-172
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659

Main Article Content

Abstract

Polyhydroxyalkanoates (PHAs) are biodegradable polymers produced by microbes as reserve material when they were cultured in a limitation of nutrients and excess C source mediums. PHAs can be synthesized by various bacteria including gram positive and gram-negative bacteria. Among the PHA-producing gram-positive bacteria, Bacillus sp. produce and accumulate various monomer compositions of PHAs. In this study, about 83,3% of strains (05/06 strains) can accumulate PHAs production. Bacillus sp. DV01 accumulated a amount of PHA 23,9% of CWD after 96h when cultured in modified basal medium containing 1% (w/v) glucose. Bacillus sp.  DV01 was identified by sequecing the gene coding for 16S rRNA. BLAST results showed the obtained 16S rRNA sequence of this strain revealed 100% with the Bacillus megaterium and so-called Bacillus megaterium DV01.

Keywords:

PHA, 16S rRNA, Bacillus megaterium DV01

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