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Research for toxicity and determination IC50 of tea leaf extracts on cervical cancer cell and breast cancer cell MCF7 by in vitro

Nguyen Thi Van Anh Nguyen Duc Thanh
Published 06/15/2020

Article Details

How to Cite
Nguyen Thi Van Anh, Nguyen Duc Thanh. "Research for toxicity and determination IC50 of tea leaf extracts on cervical cancer cell and breast cancer cell MCF7 by in vitro". Vietnam Journal of Food Control. vol. 3, no. 2 (en), pp. 80-86, 2020
PP
80-86
Counter
706

Main Article Content

Abstract

After extracting tea leaves with methanol 80% (acidified with HCl), the extraction was purified through two phases: the first stage used the column of silica gel with gradient elution, the second stage continued to be isolated on Sephadex LH-20 column with isometric elution mode, K powder was obtained. The test of toxicity of K powder on two MCF37 breast cancer cell lines and Hela cervical cancer was carried out and obtained the following results: IC50 of the composition with the line MCF37 was 31.62 µg/mL and with the line Hela was 50.1 µg/mL. Compared with the positive control group of Taxol showed that the efficacy of the obtained product was lower. However, this is a new direction in the use of drugs derived from plants in general and tea leaves in particular.

Keywords:

tea leaves, cervical cancer, breast cancer, Hela, MCF7, in vitro

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