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Isolation and identification of Bifidobacterium spp. from infant intestinal tract

Do Thi Men Pham Thi Le Tran Van Tuan Ninh Thị Tuyet Lan Nguyen Thi Minh Huyen
Received: 05 Mar 2020
Revised: 30 Jun 2020
Accepted: 30 Jun 2020
Published: 30 Jun 2020

Article Details

How to Cite
Do Thi Men, Pham Thi Le, Tran Van Tuan, Ninh Thị Tuyet Lan, Nguyen Thi Minh Huyen. "Isolation and identification of Bifidobacterium spp. from infant intestinal tract". Vietnam Journal of Food Control. vol. 3, no. 2 (en), pp. 125-132, 2020
PP
125-132
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1022

Main Article Content

Abstract

As one of probiotic bacteria, Bifidobacterium is supplement in food industry such as in milk and yogurt. The advantageous impact of these bacteria for human health was reported. However, in Vietnam, supplement of these bacteria as probiotic source to the food was not much in food industry. In our experiment, Bifidobacterium are isolated from a feces sample of 7 days-child which only had mother milk. The bacterial isolated from infant intestinal tract was Bifidobacterium infantis (B. infantis, closest to Bifidobacterium longum ssp. infantis strain Bi-26 with gene bank accession CP054425.1). The techniques to identify these bacteria were MALDI Biotyper, PCR and sequencing. This experiment is original for further study the characters of these bacteria to apply them in our daily life.

Keywords:

Bifidobacterium, isolation, identification, intestinal tract

References

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