Bìa tạp chí


Isolation and identification of Bifidobacterium spp. from infant intestinal tract

Published 06/30/2020

Article Details

How to Cite
Do Thi Men, Pham Thi Le, Tran Van Tuan, Ninh Thị Tuyet Lan, Nguyen Thi Minh Huyen. "Isolation and identification of Bifidobacterium spp. from infant intestinal tract". Vietnam Journal of Food Control. vol. 3, no. 2 (en), pp. 125-132, 2020

Main Article Content


As one of probiotic bacteria, Bifidobacterium is supplement in food industry such as in milk and yogurt. The advantageous impact of these bacteria for human health was reported. However, in Vietnam, supplement of these bacteria as probiotic source to the food was not much in food industry. In our experiment, Bifidobacterium are isolated from a feces sample of 7 days-child which only had mother milk. The bacterial isolated from infant intestinal tract was Bifidobacterium infantis (B. infantis, closest to Bifidobacterium longum ssp. infantis strain Bi-26 with gene bank accession CP054425.1). The techniques to identify these bacteria were MALDI Biotyper, PCR and sequencing. This experiment is original for further study the characters of these bacteria to apply them in our daily life.


Bifidobacterium, isolation, identification, intestinal tract


[1] T. Matsuki, K. Watanabe, R. Tanaka and H. Oyaizu, “Rapid identification of human intestinal bifidobacteria by 16S rRNA­targeted species­ and group­specific primers”, FEMS Microbiology Letters, vol. 167, no.2, pp.113­121, 1998.
[2] T. Matsuki, K.Watanabe, R.Tanaka, M. Fukuda and H. Oyaizu, “Distribution of bifidobacterial species in human intestinal microflora examined with 16S rRNAgene­targeted speciesspecific primers”, Applied and Environmental Microbiology, vol. 65, no. 10, pp 4506­4512, 1999.
[3] B. Schulthess, G. V. Bloemberg, R. Zbinden, E. C. Böttger and M. Hombach, “Evaluation of the bruker MALDI biotyper for identification of gram­positive rods: Development of a diagnostic algorithm for the clinical laboratory”, Journal of Clinical Microbiology, vol. 52, no. 4, pp. 1089 ­ 1097, 2014.
[4] MALDI Biotyper® CA System, Clinical Application for Identification of Microorganisms. BRUKER application.
[5] M. Tanaka and J. Nakayama, “Development of the gut microbiota in infancy and its impact on health in later life”, Allergology International, vol. 66, no. 4, pp. 515­522, 2017.
[6] NCBI, Bifidobacterium longum subsp. infantis strain Bi­26 chromosome. GenBank: CP054425.1 [online]https://www.ncbi.nlm.nih.gov/nucleotide/CP054425.1?report=genbank&log$=nuclalign&blast_rank=1&RID=G1Y49KXK014) [Accessed 30/3/2020].
[7] NCBI, Bifidobacterium longum Taxonomy ID: 216816 [online] https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=info&id=216816 [Accessed 30/3/2020].
[8] Y. Li, M. Shan, Z. Zhu, X. Mao, M. Yan, Y. Chen, Q. Zhu, H. Li and B. Gu, “Application of MALDI­TOF MS to rapid identification of anaerobic bacteria”, BMC Infectious Diseases, vol. 19, no.941, 2019.
[9] D. G. Hoover, “Bifidobacterium” Reference Module in Food Science: Encyclopedia of Food Microbiology (Second Edition), Pages 216­222, 2014
[10] K. C. Anukam and G. Reid, “Probiotics: 100 years (1907­2007) after Elie Metchnikoff’s Observation”, Communicating Current Research and Educational Topics and Trends in Applied Microbiology, pp. 466 ­ 474, 2007.
[11] M. Bermudez­Brito, J. P­D. S. Muñoz­Quezada, C. Gómez­Llorente, A. Gil, “Probiotic Mechanisms of Action”, Annals of Nutrition & Metabolism, vol. 61, no.2, pp. 160­174, 2012.
[12] M. Bazanella, T. V. Maier, T. Clavel, I. Lagkouvardos, M. Lucio, M. X. Maldonado­Gòmez, C. Autran, J. Walter, L. Bode, P. Schmitt­Kopplin and Dirk Haller, “Randomized controlled trial on the impact of early­life intervention with bifidobacteria on the healthy infant fecal microbiota and metabolome”, The American Journal of Clinical Nutrition, vol. 106, no. 5, pp. 1274­86, 2017.
[13] A. O’Callaghan and D. van Sinderen, “Bifidobacteria and their role as members of the human gut microbiota”, Frontiers in Microbiology, vol. 7, no. 925, 2016.
[14] R. Tanaka and M. Mutai, “Improved Medium for Selective Isolation and Enumeration of Bifidobacterium”, Applied and Environmental Microbiology, vol. 40, no. 50, pp. 866­869, 1980.
[15] M. Hadadji, R. Benama, N. Saidi, D. E. Henni, and M. Kihal. “Identification of cultivable Bifidobacterium species isolated from breast­fed infants’ feces in West­Algeria”, African Journal of Biotechnology, Vol. 4 (5), pp. 422­430, 2005.
[16] Nguyen Thi Tuyen, Isolation of Bifidobacterium from several available environments, Study project 2012, Microbiology and Biotechnology Institute, Hanoi National University.
[17] Nguyen Hoang Tuan, Isolation and selection of Bifidobacteria, application in probiotics production, Thesis for Master of Science, 2017, Hanoi National University.