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Simultaneous HPLC determination of coumarin, cinnamyl alcohol, cinnamaldehyd, acid cinnamic, eugenol, cinnamyl acetat, acid 2-hydroxycinnamic in cinnamon using HPLC

Le Viet Ngan Le Dinh Chi Nguyen Thi Hong Anh Nguyen Bich Ngoc Nguyen Thi Phuong Lan Nguyen Thi Minh Loi Le Thi Hong Hao
Published 03/10/2020

Article Details

How to Cite
Le Viet Ngan, Le Dinh Chi, Nguyen Thi Hong Anh, Nguyen Bich Ngoc, Nguyen Thi Phuong Lan, Nguyen Thi Minh Loi, Le Thi Hong Hao. "Simultaneous HPLC determination of coumarin, cinnamyl alcohol, cinnamaldehyd, acid cinnamic, eugenol, cinnamyl acetat, acid 2-hydroxycinnamic in cinnamon using HPLC". Vietnam Journal of Food Control. vol. 3, no. 1, pp. 11-19, 2020
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11-19
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Main Article Content

Abstract

The aim of the present study was the development and validation of a simple, precise and specific reversed phase HPLC method for the simultaneous determination of coumarin, cinnamyl alcohol, cinnamaldehyd, acid cinnamic, eugenol, cinnamyl acetat, acid 2-hydroxycinnamic in cinnamon. The substances were extracted from the sample matrix with methanol, shaken at room temperature for 30 minutes and determined by HPLC-PDA on the following conditions: column C18 sunfire (250 × 4.6 mm, 5 µm), mobile phase 30mM ammonium acetate and methanol-acetonitrile (50:50) using gradient. The recovery > 90%, the repeatability has RSD < 7.3%, the LODs and LOQs of2-hydroxycinnamic acid, coumarin, cinnamaldehyd, cinnamyl alcohol, eugenol were 1 µg/g and 3.3 µg/g, respectively; the LODs and LOQs of cinnamic acid, cinnamyl acetate were 2 µg/g and 6.7 µg/g, respectively. The method has been applied to analyze the samples.

Keywords:

oumarin, cinnamyl alcohol, cinnamaldehyd, acid cinnamic, eugenol, cinnamyl acetate, acid 2-hydroxycinnamic, HPLC, PDA, cinnamon

References

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