Method validation for simultaneous quantification of whey proteins in dietary supplements by HPLC

Published 01/28/2019

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How to Cite
Nguyen Thi Hong Ngoc, Mac Thi Thanh Hoa, Vu Thi Thanh An, Pham Thi Thanh Ha, Cao Cong Khanh, Le Thi Hong Hao. "Method validation for simultaneous quantification of whey proteins in dietary supplements by HPLC". Vietnamese Journal of Food Control. vol. 2, no. 1, pp. 32-38, 2019
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32-38
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Abstract

A HPLC method has been validated for simultaneous determination of Alpha-lactalbumin (α- LA) and Beta- lactoglobulin (β-LG) contents in dietary supplements by HPLC-PDA. The method was carried out in C18 column with a gradient of 0.1% TFA/ water – 0.1% TFA/ acetonitrile as mobile phase. The proteins were detected at 215 nm wavelength within 50 minutes. The method was validated in specificity, linearity, precision and accuracy; and then was applied to analyze several random dietary supplements.

Keywords:

Alpha-lactalbumin (α-LA), Beta-lactoglobulin (β-LG), whey, whey protein, dietary supplement, HPLC, PDA

References

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