Determination of rutin content in sophora japonia L. Extracts used as raw material in supplements by high performance liquid chromatography

Published 09/10/2019

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Nguyen Thanh Dat, Nguyen Thi Hong Hanh, Dam Thi Thu, Chu Thi Thuy, Hoang Thi Nuong. "Determination of rutin content in sophora japonia L. Extracts used as raw material in supplements by high performance liquid chromatography". Vietnamese Journal of Food Control. vol. 2, no. 3, pp. 51-55, 2019

Main Article Content


An HPLC-UV method was proposed in order to determine Rutin in Sophora japonica L. extract samples (3 samples extracted with 30% ethanol, 3 samples extracted with 70% ethanol and 3 samples extracted with water). The chromatographic conditions were: Agilent Zorbax Eclipse XDB C18 Column (150 x 4.6 mm, 5 µm); UV-Vis detector (257 nm); mobile phase: a mixture of methanol and 1% acetic acid solution (40:60, v/v). The linear range was of 4.97 - 298.47µg/mL; the LOQ was of 0.205 µg/mL and the accuracy was within 99.87% - 102.3%. The method was proved to be suitable for the determination of Rutin in Sophora japonica L. extracts. Rutin content in samples varied from 30.14% to 38.67%.


Sophora japonica L. extracts,HPLC, Rutin


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