Simultaneous determination of six auxin plant growth promoters in agricultural products by liquid chromatography tandem mass spectrometry (LC-MS/MS)

Published 06/15/2020

Article Details

How to Cite
Vu Ngoc Tu, Dang Thi Hien, Nguyen Thi Anh Huong, Pham Thi Ngoc Mai, Hoang Quoc Anh, Bui Cao Tien, Bui Quang Dong, Le Thi Hong Hao, Tran Cao Son. "Simultaneous determination of six auxin plant growth promoters in agricultural products by liquid chromatography tandem mass spectrometry (LC-MS/MS)". Vietnamese Journal of Food Control. vol. 3, no. 2 (en), pp. 71-79, 2020
PP
71-79
Counter
56

Main Article Content

Abstract

A highly sensitive and specific method for simultaneous determination of six synthetic auxins (4-chlorophenoxyacetic acid (4-CPA), 2-methyl-4-chlorophenoxyacetic acid (MCPA), 1-naphthalene acetic acid (NAA), 2-napthoxyacetic acid (NOA), indole-3-propionic acid (IPA), indole-3-butyric acid (IBA)) were developed using a QuEChERS-based sample extraction followed by an LC-MS/MS analysis. The method was validated and proved as meeting the AOAC International requirements for specificity, sensitivity, and accuracy in the range of 3 and 200 µg/kg. LOD and LOQ of all analytes were at 1 µg/kg and 3 µg/kg, respectively. The recoveries of six synthetic auxins were from 78.9% to 96.4% and the repeatability relative standard deviations were below 12.6%. The method was applied to simultaneously determine the content of 4-CPA, MCPA, NAA, NOA, IPA, IBA in vegetables, tubers, and fruits samples collected from different markets in Hanoi (Mai Dich, Dan Phuong, Giap Bat, Bach Mai, and Nga Tu So). MCPA was detected in 22/30 vegetable samples (3.0 ÷ 7.1 µg/kg), 9/30 tuber samples (3.2 ÷ 3.7 µg/kg); 4-CPA was detected in bean sprouts in the range from 23 to 75 µg/kg which exceeded the Maximum Residue Levels (MRL) of 10 µg/kg set by European Commission.

Keywords:

4-chlorophenoxyacetic acid (4-CPA), 2-methyl-4-chlorophenoxyacetic acid (MCPA), 1-naphthalene acetic acid (NAA), 2-napthoxyacetic acid (NOA), indole-3-propionic acid (IPA), indole-3-butyric acid (IBA), LC-MS/MS, QuEChERS, auxin, vegetables, tuber, fruit

References

[1]. Hồng Hải, “Nhập viện vì ăn ổi vừa phun chất kích thích tăng trưởng,” 14/02/2016. [Trực tuyến]. https://dantri.com.vn/suc­khoe/nhap­vien­vi­an­oi­vua­phun­chat­kich­thich­tangtruong­20160214191811562.htm [Truy cập: 20/05/2020].
[2]. Mai Loan, “Thu giữ 80.000 lọ thuốc kích thích giá đỗ từ Trung Quốc,” 15/11/2016. [Trực tuyến]. https://www.doisongphapluat.com/dia­phuong/mien­bac/thu­giu­80000­1o­thuockich­thich­gia­do­tu­trung­quoc­a9091.html [Truy cập: 20/05/2020].
[3]. Y. Bai, F. Du and H. Liu, “Determination strategies of phytohormones: recent advances”, Analytical Methods, vol. 2, pp. 1867­1873, 2010.
[4]. Y. Chen Z. Guo, X. Wang, C. Qiu, “Sample prepartion”, Journal of Chromatography A, vol. 1184, pp. 191­219, 2008.
[5]. European Commision, Directorate General for Health and Food Safety, “Analytical Quality Control and Method Validation Procedures for Pesticide Residues in Food and Feed”, Sante/12682/2019, Implemented by 01.01.2020.
[6]. H. M.G. van der Werf, “Assessing the impact of pesticides on the enviroment”, Agriculture, Ecosystems & Enviroment, vol. 60, pp. 81­96, 1996.
[7]. M. I. A. Flores, R. Romero­González, A. G. Frenich, J. L. M. Vidal, “QuEChERS­based extraction procedure for multifamily analysis of phytohormones in vegetables by UHPLCMS/MS”, Journal of Separation Science, vol. 34, no. 13, pp. 1517­1524, 2011.
[8]. L. Ma , H. Zhang, W. Xu, X. He, L. Yang, Y. Luo and K. Huang, “Simultaneous Determination of 15 Plant Growth Regulators in Bean Sprout and Tomato with Liquid Chromatography­Triple Quadrupole Tandem Mass Spectrometry”, Food Analytical Methods, vol. 6, pp. 941­951, 2013.

 Submit