Bìa tạp chí

 

009bet

Simultaneous determination of phytoestrogens in dietary supplements by high-performance liquid chromatography

Vu Thi Thanh An Mac Thi Thanh Hoa Cao Cong Khanh
Published 09/16/2021

Article Details

How to Cite
Vu Thi Thanh An, Mac Thi Thanh Hoa, Cao Cong Khanh. "Simultaneous determination of phytoestrogens in dietary supplements by high-performance liquid chromatography". Vietnam Journal of Food Control. vol. 4, no. 3, pp. 190-198, 2021
PP
190-198
Counter
583

Main Article Content

Abstract

The bio-active phytoestrogen compounds (puerarin, daidzin, glycitin, genistin, miroestrol, daidzein, glycitein, genistein) in dietary supplements were extracted by ultrasonic method with methanol solvent in 40oC. The analysis procedure was carried out on HPLC Alliance e2695 (Waters) system, with RP-C18 Reliant (25 mm × 4.6 mm; 5µm) column, at 30oC. Phytoestrogens were separated by using gradient elution with a mobile phase consisting 0.1% (v/v) phosphoric aqueous solution and methanol in 45 minutes. The method was validated by determining its specificity, linear range, limit of detection, limit of quantification, repeatability and accuracy. This method was applied succesfully to determine content of Phytoestrogens in commercial dietary supplement products.

Keywords:

phytoestrogen, puerarin, daidzein, miroestrol, HPLC, dietary supplement.

References

[1]. P. G. Aaron and W. C. Mark, “Improved methods for the extraction and analysis of isoflavones from soy-containing foods and nutritional supplements by reversed-phase high-performance liquid chromatography and liquid chromatography-mass spectrometry,” Journal of Chromatography A, vol. 913, no. 1, 2, pp. 397-413, 2001.
[2]. Association of Official Analytical Chemists, “AOAC Guidelines for Single Laboratory Validation of Chemical Methods for Dietary Supplements and Botanicals,” 2002.
[3]. A. Aresta, P. Cotugno, F. Massari, and C. Zambonin, “Determination of Isoflavones in Soybean Flour by Matrix Solid-Phase Dispersion Extraction and Liquid Chromatography with UV-Diode Array Detection,” Journal of Food Quality, vol. 2017, Article ID 8049039, 2017.
[4]. S. Malaivijitnond, K. Chansri, P. Kijkuokul, N. Urasopon, and W. Cherdshewasart, “Using vaginal cytology to assess the estrogenic activity of phytoestrogen-rich herb,” Journal of Ethnopharmacol, vol. 107, no. 3, pp. 354-360, 2006.
[5]. F. P. Pedro and C. J. Gonçalo, “Structural Analysis of Flavonoids and Related Compounds - A Review of Spectroscopic Application,” In Book - Phytochemicals - A Global Perspective of Their Role in Nutrition and Health, pp. 33-56, 2012.
[6]. J. M. Sun, B. L. Sun, F. X. Han, S. R. Yan, H. Yang, and K. Akio, “Rapid HPLC Method for Determination of 12 Isoflavone Components in Soybean Seeds,” Agricultural Sciences in China, vol. 10, no. 1, pp. 70-77, 2011.

 Submit