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Simultaneous determination of methanol, ethanol and isopropanol in human blood and white spirit samples

Vu Anh Phuong Nguyen Thi Ngan Do Thi Trang Dinh Son Luong Nguyen Thi Anh Huong Hoang Quoc Anh
Published 12/21/2020

Article Details

How to Cite
Vu Anh Phuong, Nguyen Thi Ngan, Do Thi Trang, Dinh Son Luong, Nguyen Thi Anh Huong, Hoang Quoc Anh. "Simultaneous determination of methanol, ethanol and isopropanol in human blood and white spirit samples". Vietnam Journal of Food Control. vol. 3, no. 4 (en), pp. 231-237, 2020
PP
231-237
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814

Main Article Content

Abstract

A simple and cost-effective method for simultaneous determination of methanol, ethanol, and isopropanol in human blood and white spirit samples using headspace gas chromatography coupled with flame ionization detection (HS-GC-FID) was developed and validated for clinical and toxicological purposes. In this study, the headspace sampling procedure was investigated, indicating optimized temperature of 70 °C in 20 min. Concentrations of target compounds were determined by internal standard (IS) method with acetonitrile as IS compound. A good resolution of chromatographic peaks was achieved. The linear ranges for all the compounds were from 1 to 500 mg/dL. Our method was validated with adequate accuracy (recovery > 98%) and precision (RSD < 0.1%) in whole human blood and white spirit samples. The limits of detection were below 0.5 mg/L for the three compounds. This method is easy to perform, making it suitable for routine analysis in clinical biochemistry and forensic laboratories.

Keywords:

Methanol, ethanol, isopropanol, blood, white spirit, GC-FID

References

[1]. L. O. Péres, R. W. C. Li, E. Y. Yamauchi, R. Lippi, J. Gruber, "Conductive polymer gas sensor for quantitative detection of methanol in Brazilian sugar-cane spirit", Food Chemistry, 130, 1105 – 1107, 2012.
[2]. AOAC, White spirit s. In Official methods of analysis of AOAC international, 15th Edition, pp. 739–750, 1990.
[3]. C.C. Kuo, Y.H. Wen, C.M. Huang, H.L. Wu, S.S. Wu, “A removable derivatization HPLC for analysis of methanol in Chinese liquor medicine”, Journal of Food and Drug Analysis, 10, 101–106, 2002.
[4]. K. Lynam, Screen Beer by GC/MS Static Headspace with the Agilent J&W DB-624 Ultra Inert Capillary Column, Agilent Technologies.
[5]. P. F. Pereira, R.M.F. Sousa, R.A.A. Munoz, E.M. Richter, "Simultaneous determination of ethanol and methanol in fuel ethanol using cyclic voltammetry", Fuel, 103, 725 – 729, 2013.
[6]. A. Shishov, A. Penkova, A. Zabrodin, K. Nikolaev, M. Dmitrenko, S. Ermakov, A. Bulatov, "Vapor permeation-stepwise injection simultaneous determination of methanol and ethanol in biodiesel with voltammetric detection", Talanta, 148 (Supplement C), 666-672, 2016.
[7]. B.J. Savary, A. Nuñez, "Gas chromatography–mass spectrometry method for determining the methanol and acetic acid contents of pectin using headspace solid-phase microextraction and stable isotope dilution", Journal of Chromatography A, 1017, 151 – 159, 2003.
[8]. G. C. Zhuang, Y. S. Lin, M. Elvert, V. B. Heuer, K. U. Hinrichs,"Gas chromatographic analysis of methanol and ethanol in marine sediment pore waters: Validation and implementation of three pretreatment techniques", Marine Chemistry, 160, 82 -90, 2014.
[9]. M. L. Wang, J. T. Wang, Y. M. Choong, "A rapid and accurate method for determination of methanol in alcoholic beverage by direct injection capillary gas chromatography", Journal of Food Composition and Analysis, 17, 187 – 196, 2004.
[10]. S. Kage, K. Kudo, H. Ikeda, N. Ikeda, "Simultaneous determination of formate and acetate in whole blood and urine from humans using gas chromatography–mass spectrometry", Journal of Chromatography B, 805, 113 – 11, 2004.
[11]. M. Bursova, T. Hlozek and R. Cabala, “Simultaneous Determination of Methanol, Ethanol and Formic Acid in Serum and Urine by Headspace GC-FID”, Journal of Analytical Toxicology, 39, 741 –745, 2015.

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