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Production and Purification of IgY antibodies from egg yolk

Tran Thi Sao Mai Dang Thi Oanh Dang Thi Huong Le Thanh Long Nguyen Thi Lan Huong
Published 09/25/2020

Article Details

How to Cite
Tran Thi Sao Mai, Dang Thi Oanh, Dang Thi Huong, Le Thanh Long, Nguyen Thi Lan Huong. "Production and Purification of IgY antibodies from egg yolk". Vietnam Journal of Food Control. vol. 3, no. 3, pp. 192-195, 2020
PP
192-195
Counter
470

Main Article Content

Abstract

Polyclonal antibodies from vaccinated mammalian have made a significant contribution to scientific research and diagnosis. The fact that recent technologies allow the production of antibodies in egg yolks laid by hens has led to the development of an alternative to antibody production that is less dangerous to animals. The results of this study showed that it is possible to produce a large amount of IgY antibody to bovine serum albumin (BSA), content of about 8 mg/mL of egg yolk emulsion. The anti-BSA IgY antibodies with high purity and anti-BSA activity.

Keywords:

IgY, production, egg yolk, purification, bovine serum albumin

References

[1]. R. Schade, E. G. Calzado, R. Sarmiento, P. A. Chacana, J. Porankiewicz-Asplund and H. R. Terzolo, “Chicken egg yolk antibodies (IgY-technology) - A Review of progress in production and use in research and human and veterinary medicine”, Alternatives to Laboratory Animals: ATLA, vol. 33, no.2, pp.129-154, 2005.
[2]. P. Hodek and M. Stiborová, “Chicken Antibodies - Superior Alternative for Conventional Immunoglobulins”, Proc Indian Natn Sci Acad, B69, no.4, pp.46-468, 2003.
[3]. C. Marcq, A. Théwis, D. Portetelle and Yves Beckers, “Refinement of the production of antigenspecific hen egg yolk antibodies (IgY) intended for passive dietary immunization in animals. A review”, Biotechnology, Agronomy, Society and Environment, vol.17, no.3, pp 483-493, 2013.
[4]. L. S. Munhoz, G. D’Ávila Vargas, G. Fischer, M. de Lima, P. A. Esteves, S. de Oliveira Hübner, “Avian IgY antibodies: characteristics and applications in immunodiagnostic”, Ciência Rural, vol.44, no.1, pp.153-160, 2014.
[5]. Thermo Fisher Scientific, “Thermo Scientific Pierce Antibody Production and Purification”, Technical Handbook 2, pp.1-776, vi.scribd.com/document/69292881/Radial-Immunodiffusion, 2010.
[6]. Agro-Bio, “Protocol for the production of chicken polyclonal antibodies specific IgY”, Version 2011/01.
[7]. P. Hodek, P. Trefil, J. Simunek, J. Hudecek and M. Stiborova, “Optimized protocol of chicken antibody (IgY) purification providing electrophoretically homogenous preparations”, International Journal of Electrochemical Science, vol.8, pp.113-124, 2013.

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